Carryover Problems in HPLC

If you are running a gradient, it may be beneficial to increase the amount of organic at the end of the run to ensure everything is removed from the system. Maybe it would also make sense to use a stronger mobile phase for doing the needle wash. Ensure that the column, mobile phase, and system have not been contaminated.

If you are running a gradient, it may be beneficial to increase the amount of organic at the end of the run to ensure everything is removed from the system. Maybe it would also make sense to use a stronger mobile phase for doing the needle wash. Ensure that the column, mobile phase, and system have not been contaminated.