• State Verified Answer
  • +1 person also asked this people also asked this
  • Replies 2 replies
  • Subscribers 93 subscribers
  • Views 1563 views
  • Users 0 members are here
Related

Agilent Knowledge Portal

Find maintenance, best practice, and troubleshooting articles.

Agilent Knowledge Portal

How do I lower my signal in my DAD detector in my Agilent 1260 HPLC system?

kevinqHPLC

I am setting up our new HPLC for preparative scale separation.

Currently, I am saturating the UV-detector (the peaks in the chromotagram are cut-off at the top). 

How do I lower signal intensity? I tried changing the attenuation value but this had no effect on the signal intensity. 

I apologize if this is a trivial issue. 

Best,

Kevin

Parents
  • +1

    Hello,

    You will need to modify the sample concentration [less concentration] or make a smaller injection volume.  Alternatively, you can choose a wavelength where it absorbs less intensely and that will lower the signal as well.

    I assume you are collecting the signal into a PC digitally and if true, lowering the attenuation will have no effect.  Attenuation is for analog signal manipulation.

Reply
  • +1

    Hello,

    You will need to modify the sample concentration [less concentration] or make a smaller injection volume.  Alternatively, you can choose a wavelength where it absorbs less intensely and that will lower the signal as well.

    I assume you are collecting the signal into a PC digitally and if true, lowering the attenuation will have no effect.  Attenuation is for analog signal manipulation.

Children
No Data
Was this helpful?
Privacy Statement
Terms of Use
Contact Us
Site Help