I am looking for information that would explain a significant difference in peak areas between G1321B (Agilent 1260 Infinity) and G7121B (Agilent 1260 Infinity II) FLDs. The same method and samples are being analyzed on both HPLCs, LU response is higher on G7121B (0.35 LU) than that on G1321B (0.05 LU), but peak areas are significantly smaller on G7121B (e.g., 2 uV*sec.) versus G1321B (e.g., 294344 uV*sec). Initially it was thought that this was caused by an analog output range setting of 0.1V versus 1V, however, it was not the case since G7121B had been verified as being set at 1V. What causes this peak area difference?
Maybe it is still due to the analog output range that may have a different setting in Empower configuration? Empower configuration will override settings on the instrument, if they're not a part of the instrument method. Analog voltage range was a part of the instrument method on G1321A (Agilent 1100), but not anymore on Agilent 1260s. In any case, what does this setting do? How does it impact the peak area?
Many Thanks for your help!