1260 SFC Maximum modifier concentration

Question

Using a 1260 SFC, not a hybrid system, what is the maximum %B (modifer) that I can run? Methods seem to go as high as 50% modifier 
 Thanks in advance!

dannymobbs · Accepted Answer

Typically, I advise customers to set the composition to 100% co-solvent (MeOH normally) and then flush the column for a couple of minutes. As stated before, the BPR most likely will not be totally stable at 100% MeOH but just ignore that. After 10-20 column volumes of co-solvent turn off the flow and de-pressurise the system. You referred to the vent so you know when to find this setting. After de-pressurisation, remove the fitting from the front of the column and then the back. You will likely still see solvent spitting or fizzing out of the column even after depressurising. If you want to be totally sure to store the column in solvent, the only real way to do this is to flush it from an LC pump (if you have a free system in the lab).

1260 SFC Maximum modifier concentration

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