I also changed the column and guard cartridge too. This didn't help either. Any suggestions would be most welcome. TIA!
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I also changed the column and guard cartridge too. This didn't help either. Any suggestions would be most welcome. TIA!
Without knowing what model of DAD you have, what I can say is if it is noisy, I would try another flow cell if I have one. Make new mobile phase (if you just dump mobile phase in the bottle every time, change solvent bottle and make fresh mobile phase. I would also look at my pressure to make sure my pressure is stable or if it fluctuates a lot. If it fluctuates, look into your check valves maybe or your degaser.
Without knowing what model of DAD you have, what I can say is if it is noisy, I would try another flow cell if I have one. Make new mobile phase (if you just dump mobile phase in the bottle every time, change solvent bottle and make fresh mobile phase. I would also look at my pressure to make sure my pressure is stable or if it fluctuates a lot. If it fluctuates, look into your check valves maybe or your degaser.
Pump pressure is something I routinely monitor - pressure has been solid. Will try the cell.