I have several questions related to interpreting the results from injecting the DB-5 standard into my 7890B GC/5977B MS. 1.) are the 8 analytes supposed to elute in the order specified on the Certificate of Analysis? 2.) If they elute in a different order, is that meaningful in any way or does it indicate anything regarding the "health" of the column? 3.) Masshunter Qual didn't identify three of the 8 peaks. I manually integrated the 3 then did a NIST library search. The three compounds did elute, however the library search match for them was very low, < 30%. Is this meaningful at all? 4.) In the end, I'm not quite clear on what I'm supposed to glean from running the standard. I will admit, I'm running it after having used this particular column for some time. 5.) Is the goal of running the standard simply that I'm meant to compare changes to the peak intensities and locations with time? 6.) How do I harness the data to figure out if I need to trim the column or install a new one? PHEW! That's a lot of questions. Any help/guidance is appreciated. Thank you.
