EPA 552.3 HAA Method

I have a quick question about the EPA 552.3 HAA method and doing a dilution. In the method it states that I can dilute the extract with the MTBE with the internal standard. When I run the diluted sample how would I account for the increased concentration of the internal standard? Or would the increased concentration not even make a difference if for example I do a 1:3 dilution with a final volume of 0.6mL?

Any help with this will be appreciated!

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