Is Hypersil AA-ODS 79916AA-572 column actually an (AC) column?

Hi, everyone!

Is Hypersil AA-ODS 79916AA-572 column actually an (AC) column?

I can see on a web site it is:

If it is I should use in-column-hardware-fit guard cartridges 7992618-504 Hypersil ODS, 5 µm, 4 x 4 mm cartridge (AC), 10/pk (use with hardware kit 5021-1845).

The question arised as I can see elsewhere a totally different information, it is standalone column and a standalone Thermo-style guard holder (Female-Female, connecting capillary required) with cartridges:

And it looks like a standalone column on ebay:

AGILENT Hypersil AA-ODS 200 x 2.1mm 5µm Particles HPLC Column 79916AA-572 | eBay

  • It seems I saw a 5063-6588 kit not so long ago, the column 79916AA-572 was standalone:

  • Thank you for your answer, I would never use legacy Aminoquant method Hypersil AA-ODS myself and never suggest it to any person living in 2019. It is a kludge method.

    The deal is it is not for me and I'm not allowed to change their workflow. It's not just a hardware, it is also about guards. I should fit in an existing workflow.

    It is cheaper not to return anything from the country I'm living, it is close to impossible.

  • Full disclosure, I am an Agilent sales person, but am posting this as a member of the community, not an official Agilent expert.  It is my experience I would trust a company website over ebay.  From what I can tell, Hypersil is not an Agilent particle, ie we don't manufacture it, we sell it as a service to our customers who still use them or need them, for example in a validated method.  If you are required to use this column, I would suggest buying the column using the part numbers on the Agilent website (column and hardware kit).  If we did make a mistake in the description, you can always return the hardware.  Another option would be to talk to our application support team in your country (I work out of the Midwest in the U.S. so my application support email is LC-column-support@agilent.com)  and ask them for other options on more modern particles, for example solid core poroshell HPH, which is designed to run at the high pHs that are commonly used in some AA analyses.  We have also recently introduced a new AdvancedBio Amino Acid column, you can find information about that here: https://www.agilent.com/en/products/liquid-chromatography/lc-columns/biomolecule-separations/advancebio-amino-acid-analysis-(aaa) .

  • Hi all,

     

    79916AA-572 IS a conventional column. We are aware of the typo in the online store and it will be changed as soon as possible.

     

    And due to the high pH's of amino acid analyzes Agilent's HPH columns (Poroshell 120 HPH-C18) are far better suitable for them then the AA-ODS column.

     

    Cheers,

     

    Norbert

  • Hello, Norbert! Thank you for your response.

     

    In the wild here (and on Agilent application notes on AAA as well) I pretty much frequently see just an Eclipse Plus C18 and XDB-C18 (or proprietary Eclipse AAA particles, sharing eXtra Dense Bonding process), and not Extend C18 to resist high pH in this application of AAA, why, if high pH-resistance is of such a great importance? Won't it be enough to change sacrificial guards regularly to cease particles in column dissolution to a practically acceptable rate?

     

    I usually advocate for a specialty phase https://www.agilent.com/en/products/liquid-chromatography/lc-columns/biomolecule-separations/advancebio-amino-acid-analy…

    even if it is an Poroshell 120 HPH-C18 column with a "proprietary phase" label attached. I hope much more work had been done on that.

  • Hi,

    amino acid analyses changed little over time, that's why historical phases are still used. Then came 2nd and 3rd generation columns with ultra-pure silica bases and better stability. Today I would always use an AdvanceBio AAA instead of anything else, but there are plenty of literature references out there citing the old ways.

    If an old type column is in a validated method, the user should calculated the financial benefits of using a state-of-the-art phase and re-validate the method and multiple older columns with short life spans.

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