I am isolating mitochondria from mouse brain and want to run seahorse onto it, could you recommend the best possible protocol for the isolated mitochondria on seahorse XFe96 analyzer?
Looking forward,
Best regards.
Gaurav
I am isolating mitochondria from mouse brain and want to run seahorse onto it, could you recommend the best possible protocol for the isolated mitochondria on seahorse XFe96 analyzer?
Looking forward,
Best regards.
Gaurav
Hi Guarav,
Thank you for contacting Cell Analysis Technical Support. We do have some resources/protocols regarding Iso Mito on the 96 platform:
Analyzing Microgram Quantities of Isolated Mitochondria in the Agilent Seahorse XFe/XF96 Analyzer
Keep in mind that the isolation of mitochondria itself will differ from species to species and cell type to cell type, so you will have to find the best method specific to mouse brain mito.
Please let me know if these resources help or you have any other specific questions!
Thank you,
Courtney
Courtney Nadeau Watts
Technical Support Scientist/Remote Engineer
Cell Analysis Products
Phone Contact: 800-227-9770 (Option 3 , option 8)
Email Contact: cellanalysis.support@agilent.com