Seahorse on isolated mitochondria

Hi Guarav,

Thank you for contacting Cell Analysis Technical Support. We do have some resources/protocols regarding Iso Mito on the 96 platform:

Analyzing Microgram Quantities of Isolated Mitochondria in the Agilent Seahorse XFe/XF96 Analyzer

Measuring Mitochondrial Function in Permeabilized Cells Using the Seahorse XF Analyzer or a Clark-Type Oxygen Electrode

Keep in mind that the isolation of mitochondria itself will differ from species to species and cell type to cell type, so you will have to find the best method specific to mouse brain mito.

Please let me know if these resources help or you have any other specific questions!

Thank you,

Courtney

Courtney Nadeau Watts

Technical Support Scientist/Remote Engineer

Cell Analysis Products

Phone Contact: 800-227-9770 (Option 3 , option 8)

Email Contact: cellanalysis.support@agilent.com

Hi Guarav,

Thank you for contacting Cell Analysis Technical Support. We do have some resources/protocols regarding Iso Mito on the 96 platform:

Analyzing Microgram Quantities of Isolated Mitochondria in the Agilent Seahorse XFe/XF96 Analyzer

Measuring Mitochondrial Function in Permeabilized Cells Using the Seahorse XF Analyzer or a Clark-Type Oxygen Electrode

Keep in mind that the isolation of mitochondria itself will differ from species to species and cell type to cell type, so you will have to find the best method specific to mouse brain mito.

Please let me know if these resources help or you have any other specific questions!

Thank you,

Courtney

Courtney Nadeau Watts

Technical Support Scientist/Remote Engineer

Cell Analysis Products

Phone Contact: 800-227-9770 (Option 3 , option 8)

Email Contact: cellanalysis.support@agilent.com