Has anyone coated the XF Flux Cell culture Plates with Matrigel to facilitate adherence of suspended cells?
Has anyone coated the XF Flux Cell culture Plates with Matrigel to facilitate adherence of suspended cells?
Hi Stephanie,
You are very welcome! I took a look and did find this paper:
Protocol to profile the bioenergetics of organoids using Seahorse
We also do have a paper ourselves using Matrigel, but it is applicable to the 24 well instrument:
The XF24 microplates have a surface area of 0.28 cm2 to the XF96 surface area of 0.11 cm2. We typically seed roughly 40% of cells per well with the XF96/XFp compared to the XF24.
Please take a look and let me know if this helps!
Thank you,
Courtney