For the Mito stress test kit protocol (kit 103015-100), it says to hydrate the sensor cartridge in xf calibrant in a 37C incubator overnight. I did this on my most recent assay and got strange results. The cells did not appear to respond to the injected drugs as strongly as they have in the past when another lab member did the same experiment.
When I clicked on the extended protocol for this portion, it has different instructions which are to incubate overnight in water and then replace with calibrant an hour before the assay. Could incubating overnight in the calibrant instead of water have adversely affected my assay?
Thank you for your help!