Hello everyone,
My team is currently seeing horrible baselines for all 96 wells of our PCR plates that we run on our two Agilent Oligo Pro II. Normally, we see this issue when the temperature in our lab gets above 72-74 degrees F. However, we have since dropped the temperature of the room to 68.6 degrees. The buffers and conditioning gels have all been changed. The CAC box has been changed and the wall plug has been changed onto another wall socket position. Both machines also have separate power sources. My team and I are completely unsure about how to solve the problem. For context, I have added pictures of the baseline fails below.