I used promega RNA ladder to confirm the instrument status (Figure 1). Is the difference in size (nt) within a reasonable range? (Figure 2)
The concentration in Figure 3 should be twice as high as in Figure 4, but the reason for the lower concentration observed in Figure 3 is what? In Figure 5, the concentration should be similar to Figure 6, but the situation presented in Figure 5 shows no signal. What could be the possible reasons?
Additional information: The RNA ladder I used is Agilent DNF-472 HS RNA (15nt)