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Videos How to Improve Peak Shape with Feed Injection in Your HPLC Autosampler
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How to Improve Peak Shape with Feed Injection in Your HPLC Autosampler

With Agilent Feed Injection, you can optimize peak shapes by gaining full control over sample injection in your HPLC autosampler. By adjusting injection speed, flush-out solvent, and flush-out volume, you can get excellent results in your LC and LC/MS analysis.
 
In this video, you will learn how Feed Injection mode compares to conventional flow-through injection mode. This guide will show you how to set up Feed Injection parameters to improve peak shapes in HPLC.

The first section of this video will demonstrate how Feed Injection mode operates differently to flow-through injection mode in HPLC.

Next, the influence of Feed Injection parameters on chromatography will be introduced. By using lower feed speeds and higher sample dilutions, Feed Injection reduces strong solvent effects and improves peak shapes. 

The video will also present how adjusting Feed Injection parameters can lead to high recovery and low carryover. 
Take me to:
  • How to Improve Peak Shape with Feed Injection in Your HPLC Autosampler (Agilent.com)
  • 1260 Infinity II Hybrid Multisampler product page
  • Frequently asked questions (FAQs) on how the Hybrid Multisampler works
  • Operation
  • 1260 Infinity II LC System
  • Best practices
  • LC Injectors and Sample Thermostats
  • Analytical LC Systems
Carlos Vargas
Carlos Vargas
  • 21 Jul 2023
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