This Information Applies To: All Agilent HPLC Autosampler modules
Nonreproducible peak areas between injections, using the same sample and the same experimental conditions.
The issue may come from different sources, it is recommended to verify all the following ones for a successful troubleshooting.
Warning: When opening capillary or tube fittings, solvents may leak out. Observe appropriate safety procedures (for example, wear goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the solvent vendor, especially when toxic, or hazardous solvents are used.
If the problem persists, contact Agilent Technologies for further guidance.
Learn how to effectively troubleshoot your LC autosampler:
HPLC-0GEN-2201e - Chromatographic Troubleshooting for HPLC e-learning course available from Agilent Education