This Information Applies To: Agilent 500 Series ICP-OES (5100, 5110, 5800, 5900)
The ICP-OES nebulizer converts the liquid sample into an aerosol as it enters the spray chamber. A dirty or partially blocked nebulizer traps sample, which leads to poor reproducibility and reduced sensitivity. Droplets on the internal surface of the spray chamber can indicate poor conversion of liquid to mist.
The ICP-OES sample introduction components are detailed in Figure 1.
Figure 1: ICP-OES sample introduction components
1. Torch loader handle 2. Torch 3.Torch clamp 4. Nebulizer gas connections 5. Spray chamber drain tubing 6. Spray chamber 7. Nebulizer 8. Nebulizer inlet
|Tips: Not all nebulizers are the same, and fittinmgs will vary with the nebulizer.|
A blocked nebulizer can be identified by a visual inspection as per Figure 2.
Figure 2: Blocked nebulizer
1. Blockage at tip 2. Sample trapped in the nebulizer tip
Steps to follow
|Caution: The concentric glass nebulizer is fragile. Handle with care. Do not place a glass nebulizer in an ultrasonic bath as vibrations may cause edge chipping or cracking and degrade the performance of the nebulizer. Do not use hydrofluoric acid with glass or quartz sample introduction components.|
Turn off the pump and the plasma. Remove the nebulizer, being careful not to touch the tip of the nebulizer.
Different cleaning solutions can be used to clean the nebulizer including 2.5% detergent solution, methanol, or 10% nitric acid. Flush the nebulizer with the selected cleaning solution in both directions:
Push liquid through using a syringe attached with a small piece of soft plastic tubing or the nebulizer cleaning tool (part number G3266-80020). This tool is suitable for concentric nebulizers. It cannot be used with the Agilent OneNeb nebulizer as it differs in construction.
Pull liquid through with a syringe attached with a small piece of soft plastic tubing to the nebulizer inlet. Block the nebulizer gas inlet side arm. Place the tip of the nebulizer in the liquid and draw the liquid through the sample inlet end.
Rinse with de-ionized water.
|Warning: Wear Personal Protective Equipment when handling nitric acid, methanol, and laboratory detergents.|
Reinstall the nebulizer.
|Recommended maintenance schedule: Agilent recommends weekly cleaning of the nebulizer. Analysis of samples with complex matrices may require more frequent cleaning.|
Learn how to effectively maintain your Agilent ICP-OES:
ICPOES-5110-1270e, Agilent 5110 ICP-OES Maintenance, and Troubleshooting e-learning course available from Agilent education