Has anyone analyzed ethoxylated C16-C18 alcohols before on HPLC-RI?
I have tried the following set up:
Column: Acclaim Surfactant Plus column 150mm*4.6mm
Mobile A: H2O Mobile B: ACN
Gradient: Start with 50%B isocratic to 100% B
Column T: 25C
Injection V: 5 ul
Flow Rate: 0.5 ml/min
I did not see a single peak. Any suggestions?