HPLC-RI analysis of ethoxylated C16-C18 alcohols


Has anyone analyzed ethoxylated C16-C18 alcohols before on HPLC-RI?

I have tried the following set up:

Column:  Acclaim Surfactant Plus column 150mm*4.6mm 

Mobile A: H2O Mobile B: ACN

Gradient: Start with 50%B isocratic to 100% B

Column T: 25C

Injection V: 5 ul

Flow Rate: 0.5 ml/min

I did not see a single peak. Any suggestions?

Thanks Slight smile

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