How can I put Relative RT on the chromatogram?!

Question

How can I put relative RT on the chromatogram?! Added from annotation it looks like are all 0.000; I don't know to establish reference peak. My version of software is

ryoboyle · Accepted Answer

Hello, I added tags to this post to increase visibility. In order to calculate the relative retention time to be displayed as a peak annotation you will need to make sure that you follow the below steps. 1) In your Advanced Method Options (accessed by going to Method>Advanced), go to the Column/Performance tab and enter in the retention time of an unretained peak. Then check the 'Calculate performance parameters for this channel' checkbox. 2) In the Peaks table you need to populate the 'Ref. ID #' column with the Peak # of the reference peak. For example, in the below screenshot Peak2 is identified as the reference peak for all other compounds. Once those two things are done, process your result set and the relative retention time should be calculated. Hopefully this helps.

How can I put Relative RT on the chromatogram?!

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