How can I put Relative RT on the chromatogram?!

How can I put relative RT on the chromatogram?!
Added from annotation it looks like are all 0.000; I don't know to establish reference peak.
My version of soft is

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  • Hello,

     

    I added tags to this post to increase visibility.

     

    In order to calculate the relative retention time to be displayed as a peak annotation you will need to make sure that you follow the below steps. 

     

    1) In your Advanced Method Options (accessed by going to Method>Advanced), go to the Column/Performance tab and enter in the retention time of an unretained peak. Then check the 'Calculate performance parameters for this channel' checkbox.

     

    2) In the Peaks table you need to populate the 'Ref. ID #' column with the Peak # of the reference peak. For example, in the below screenshot Peak2 is identified as the reference peak for all other compounds. 

     

     

    Once those two things are done, process your result set and the relative retention time should be calculated. 

     

    Hopefully this helps. 

  • Hello!

    Thank you for the answer, it was helpful, but I realiase that I need in fact for Relative Retention Time (RRT=relative retention time for impurities with respect to principal peak ), that has another formula (RRT=RT of the impurity peak /RT of the principal peak).
    The question is : Can I bring (and eventually how ) new formulas that I need in soft in order to automatically calculate and also be written in the chromatogram report?! 
    Another example is for Area% that I want to calculate like described in European Pharmacopoeia for a specific product so another formula that Area% is generated from soft.

     Thank you alot!

Reply
  • Hello!

    Thank you for the answer, it was helpful, but I realiase that I need in fact for Relative Retention Time (RRT=relative retention time for impurities with respect to principal peak ), that has another formula (RRT=RT of the impurity peak /RT of the principal peak).
    The question is : Can I bring (and eventually how ) new formulas that I need in soft in order to automatically calculate and also be written in the chromatogram report?! 
    Another example is for Area% that I want to calculate like described in European Pharmacopoeia for a specific product so another formula that Area% is generated from soft.

     Thank you alot!

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