Calibration table for antibody concentration

Hello 

I have run a number of samples of unknown concentrations for an antibody, I was given a purified antibody with known concentration to create a standard curve. When I go to create my calibration table I end up getting a warning message stating that my standards have the same peak RT. Just so I understand when creating a standard curve in OpenLab CDS do you setup a sequence run so each injection has a different amount and then associate each level to that injection amount? 

Parents Reply Children
Was this helpful?