Calibration table for antibody concentration


I have run a number of samples of unknown concentrations for an antibody, I was given a purified antibody with known concentration to create a standard curve. When I go to create my calibration table I end up getting a warning message stating that my standards have the same peak RT. Just so I understand when creating a standard curve in OpenLab CDS do you setup a sequence run so each injection has a different amount and then associate each level to that injection amount? 

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