Height peak in LC Agilent vs LC Waters

Hi Community! I'm making a peptide mapping that it's a identification assay.

The problem is that intensity in LC Agilent is not the same LC Waters.

I would like to know if exists any table with conversion factor.

Thank you!


  • Sorry this is a bit late. It's very unlikely such a thing exists. The reason being that there are too many unpredictable variables. For any change in column, method, system, detector, analytes, or solvent, you should verify system suitability and redo calibration. If your new calibration isn't significantly different from the previous and isn't trending in some way over time, then you can batch it in with the old pretty safely. Depending on the changes made, it may be completely reasonable to expect wildly different results. However, this is the reason system suitability requirements exist. You use them to ensure precision, accuracy, and repeatability despite the changes. At worst, you can make sure that system suitability passes within a singular run and calibration.

  • Hello,

    What software are you using? You cannot change the raw signal data but if you need to see the area, height, RF in different units, those are calculations you can build into the report or use custom calculator in CDS 2.x. Not sure if that is what you are looking for in this case, but it can be done. 

    Marty Adams

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