Hello,
When I try to create a new nested baseline, the baseline won't go past the middle of the peak. I need to separate an entire peak from a range of the chromatogram, does anybody know how to get around this issue?
Thanks!
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Hello,
When I try to create a new nested baseline, the baseline won't go past the middle of the peak. I need to separate an entire peak from a range of the chromatogram, does anybody know how to get around this issue?
Thanks!
Hi Dylan
Can you share a picture of the peak that you're trying to integrate? Nested baselines can't go beyond the peak apex since they are intended to be front or tail tangent skims, which shouldn't really go beyond the apex of the main peak.
/Andy
Thanks for the response Andy! The peak at 7.822 min. is a surrogate peak and needs to be integrated separately from the range that is in included in.
Hi Dylan
Thanks for sharing the chromatogram, I can see the problem clearly now. I don't think I have a good answer for you, so hopefully, someone else in the community can find an answer for you.
/Andy
Thanks for taking a look
Hello,
You would need to have the area sum window or area slice window setup so that the middle does not fall on that peak. The manual integration tool treats that middle point as it would a normal peak apex and will not let the nested baseline cross it.
Marty Adams
Thanks for the reply Marty!
I think my problem is that this peak is the highest point in the chromatogram so it's always going to be treated as the apex.
Thanks for the reply Marty!
I think my problem is that this peak is the highest point in the chromatogram so it's always going to be treated as the apex.
Hello,
Do you have an area sum window from ~7.2 to ~8.4 or is that peak at 7.822 a normal peak found by integration? If the peak at 7.822 is a normal peak not the center of an area sum window, then just delete and redraw it in manual integration. The "apex" of an area sum window or area slice is not the highest point but the middle of the time window.
Marty Adams
Oh I see what you're saying. Sometimes I need to overwrite the baseline after processing the data and manually make the splits. Only when I do this am I having the issue of it picking the highest point in the range as the apex. Maybe I'm just unable to integrate it this way?