How to convert counts values to mAU in EzChrom Elite?

Hello community

I am using EzChrom Elite. This software in the Y-Axis in order to measure response uses counts instead of mAU. In order to perform some calculations from chromatographic results I would like to know if there is a way to convert counts to mAU and viceversa. NOT SPECIFICALLY A FEATURE IN THE SOFTWARE, BUT A NUMBER WHICH I COULD USE TO MAKE THE CONVERSION MANUALLY.

I appreciate your help!

Best Regards

Parents

0 Stormbringer over 2 years ago

Hello,

Please mention the instrument you are trying to work with.

Please see the following for GC:

GC Signal Conversion Factors

				Use to convert to display scale units	Use to convert to unscaled units

	Detector Type	Scale	units	GC LSV (1/7680)	Multiplier
	FID/NPD	1	pA	0.0001302083	0.000130208
	TCD	25	uV	0.0001302083	0.003255208
	ECD	5	Hz	0.0001302083	0.000651042
	AIB	15	uV	0.0001302083	0.001953125
	uECD	1	Hz	0.0001302083	0.000130208
	FPD	150	pA	0.0001302083	0.01953125

GC drivers display their signals for each detector based on a pre-defined scale that varies based on detector type.
To convert the raw area and height counts in EZChrom standard reports to a unit based scale that matches with the
chromatogram display (i.e. 25uV for TCD) multiply any signals count value by the GC LSV (Divide counts by 7680).

To convert counts to an unscaled detector signal (i.e. uV) the LSV and Scale are factored to give a Multiplier.
Multiply detector counts by this multiplier to get unscaled signal values in units.

	Example Calculation DAD:
	Choose detector:	1100/1200 DAD (G1315)
	Scaling:	1.00E+03
	UnitConv:	0.476678277
	Units	mAU

Detector	Unit Conversion	Deffault Scaling	1 Count	Units
VWD (G1314)	5.9604644775E-05	1.00E+06	2^ -24AU	mAU
1100/1200 DAD (G1315)	4.7667827696E-01	1000	2^-21AU	mAU
1100/1200 MWD(G1365)	4.7667827696E-01	1000	2^-21AU	mAU
1100/1200 DAD (G1315) w/SP1 FW A.02.01	4.7683715820E-01	1000	2^-21AU	mAU
1100/1200 MWD(G1365) w/SP1 FW A.02.01	4.7683715820E-01	1000	2^-21AU	mAU
1200 FLD (G1321)	5.9604644775E-05	1.00E+06	2^ -24AU	LU
1200 RID (G1362)	N/A
1200 ELSD (G1428)	N/A

Reply

0 Stormbringer over 2 years ago

Hello,

Please mention the instrument you are trying to work with.

Please see the following for GC:

GC Signal Conversion Factors

				Use to convert to display scale units	Use to convert to unscaled units

	Detector Type	Scale	units	GC LSV (1/7680)	Multiplier
	FID/NPD	1	pA	0.0001302083	0.000130208
	TCD	25	uV	0.0001302083	0.003255208
	ECD	5	Hz	0.0001302083	0.000651042
	AIB	15	uV	0.0001302083	0.001953125
	uECD	1	Hz	0.0001302083	0.000130208
	FPD	150	pA	0.0001302083	0.01953125

GC drivers display their signals for each detector based on a pre-defined scale that varies based on detector type.
To convert the raw area and height counts in EZChrom standard reports to a unit based scale that matches with the
chromatogram display (i.e. 25uV for TCD) multiply any signals count value by the GC LSV (Divide counts by 7680).

To convert counts to an unscaled detector signal (i.e. uV) the LSV and Scale are factored to give a Multiplier.
Multiply detector counts by this multiplier to get unscaled signal values in units.

	Example Calculation DAD:
	Choose detector:	1100/1200 DAD (G1315)
	Scaling:	1.00E+03
	UnitConv:	0.476678277
	Units	mAU

Detector	Unit Conversion	Deffault Scaling	1 Count	Units
VWD (G1314)	5.9604644775E-05	1.00E+06	2^ -24AU	mAU
1100/1200 DAD (G1315)	4.7667827696E-01	1000	2^-21AU	mAU
1100/1200 MWD(G1365)	4.7667827696E-01	1000	2^-21AU	mAU
1100/1200 DAD (G1315) w/SP1 FW A.02.01	4.7683715820E-01	1000	2^-21AU	mAU
1100/1200 MWD(G1365) w/SP1 FW A.02.01	4.7683715820E-01	1000	2^-21AU	mAU
1200 FLD (G1321)	5.9604644775E-05	1.00E+06	2^ -24AU	LU
1200 RID (G1362)	N/A
1200 ELSD (G1428)	N/A

Children

0 ilopez59 over 2 years ago in reply to Stormbringer

Hello Stormbringer

Although the software is Agilent, the detector i'm using is not from Agilent, but a Hitachi L-7400 UV Detector.

Thank you for your response
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