Partial calibration in real time openlab CDS

Hello,

After you setup the method in your first result set did you use the update master method selection? When you link a method to a result set it copies that method into the result set and any changes you make are on that method copy in the result set. If you want to update the original master method to use when running sample then you need to use the update master method.

Marty Adams

Ok, I'm still doing something wrong (and I can't add pictures like I could yesterday for some reason)

I Updated the Master Method as shown, and assumed it updating it would save it.  I ran another batch and got the same two point calibration as before. 

So today I reprocessed that data to get all six calibration points, updated the master method, then SAVED Method (which I hadn't done before because I thought it would automatically save). 

But when I exited to go back to the sequence, I got the message "Unsaved Changes--There are unsaved results-- Do you want to proceed closing without saving?"  I couldn't find where I could save anything else because "Save Method" was already greyed out since I had just done that.

I re-ran the sequence and I am STILL getting the 2-point calibration on one column and nothing on the other.

Do I have to do something to link the method?  I don't understand the link icon.  I tried that, but it didn't work, either.

The "Unsaved changes" message refers to the results not the method.  When you reprocess data in Data Analysis, you need to save the results before you close the program, or before you generate reports.

Back to the cal. curve problem:

Have a look at the Injection List in Data Analysis.  Are all of your standards correctly identified and is Clear All calibrations set just for the first standard?

Does the processing method still have 6 levels.  Check this on the General tab of the Compounds...Calibration part of the method.

Not sure why you can't share more images.  I think I saw that another user had the same problem today.

/Andy

Ok, thanks for the information on the results.  I don't care about saving reprocessed results since my focus is to just get a method established that works the way I need it to.  As long as I don't need to do this for updating my master sequence to work.....(Looks like pic insertion is now working)

In my Injection List in Data Analysis, my standards are correctly identified, levels designated, and "clear all calibrations" is set just for the first standard:

My processing method still has all 6 levels:

I also have internal standards correctly designated along with the correct association of analytes:

Hi ALAZ

I wonder if you look at the list of injections in the navigation tree.  What errors are reported.  If you make the navigation side wider, you'll see whether these injections have some errors.

Alternatively, right-click on one of the injections and review the result set log

That should give you more of an idea about what's going on.

/Andy

Hi,

Thanks for your response....again!

Yes, I had looked at these of course.  You'll notice that I have no "warnings" or injection errors upon reviewing the data with the partial or failed calibration: 

However, once I reprocess the data with the same method....I get the warnings.  There does not appear to be any injection errors since all of the data I need is present.  The warnings are not entirely unexpected since I have several other volatiles in my method that I don't calibrate nor quantitate (methanol, isopropanol, acetone, and acetaldehyde).  I only want to calibrate and quantitate ethanol.  So I figured I should only be interested in the "ethanol" and "Ethanol" warnings. The first calibrator rightly indicates that there is only one point available for the calibration: 

The rest of the calibrators look like this with no "Ethanol" or "ethanol" warnings.  I expect the warnings about the other volatiles not being found: