FLD Wavelength accuracy and signal to noise not passing.

Question

Hi, 
 Recently Run the Wavelength accuracy and signal to noise at FLD detector for qualification purposes but it not passing. In wavelength accuracy test got Excitation value 350 it ok but in case of emission got 393 nm its failed required minimum 394. In Signal to noise got 367 required value minimum 400. Run Wavelength calibration from Labadvisor using Glycogen solution it passed but when try wavelength accuracy test from LA it aborted by system when scan emission. please help what should do next. 
 Note: I used double filter water and sonicate more than 1 Hour. 
 
 Thanks

sschombu · Accepted Answer

Apologies for the delay, but I was off for Christmas. There is no cleaning procedure. What I meant to say is that both, the lamp house window and the EX condenser (no dismantling!) could be cleaned with a lint free cotton cloth and IPA, but that would only help if there's dirt or dust. More likely there will be optical degradation, after constant bombardment with powerful UV light for years. Here cleaning does not help at all and the parts have to be replaced. No cleaning at all to be carried out on the mirror and the grating! Remember to re-calibrate the FLD, after you did any changes to the optical path, including removal of the cell. Do not carry out an intensity test before re-calibration.

FLD Wavelength accuracy and signal to noise not passing.

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