FLD Wavelength accuracy and signal to noise not passing.


Recently Run the Wavelength accuracy and signal to noise at FLD detector for qualification purposes but it not passing. In wavelength accuracy test got Excitation value 350 it ok but in case of emission got 393 nm its failed required minimum 394. In Signal to noise got 367 required value minimum 400. Run Wavelength calibration from Labadvisor using Glycogen solution it passed but when try wavelength accuracy test from LA it aborted by system when scan emission. please help what should do next.

Note: I used double filter water and sonicate more than 1 Hour.


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