contamination cannot be removed

Hello Together,

I have a contamination (12-15min): it's also there when I change the device, make new eleuents and use different columns. The eluents are 0.1% H3Po4 in water and 0.1% H3Po4 in ACN. The contamination remains stable and comes with increasing gradient B. New containers were opened, purge frits were exchanged. Does anyone else have an idea?



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