We have a system with the following parts.
- Tray with THF Bottle as solvent A/no solvent B and Waters in Line Degasser
- 1200 Series G1312A Binary Pump
- 1100 Series G1313A Autosampler
- 1100Series G1316A Column Compartment with guard column and analytical column in sequence
- 1200 Series G1362A RI Detector
It has a baseline issue, was sitting for a long time unused and fixing and replacing the following things did not improve it:
- Both solvent lines are drawing solvent from the same bottle
- replaced solvent filters
- hooked the system up properly (channel B was not hooked up at all and the capillaries were connected wrong)
- changed the pump seals and did a pump-wear in procedure
- cleaned the pistons and the inside of the pump
- exchanged the active inlet valve and the outlet valve on both pump heads
- exchanged the cartridge in the purge valve
- primed degasser and pumps with IPA
I am currently bypassing columns for testing and the baseline shows a lot of noise. I am currently trying to clean the flow cell as I was told that this may be the issue. I would like to open the flow cell, but cannot find a description of how to do this in the manual.
It says that the maximum pressure of 5 bar should not be exceeded for the flow cell, but since I did not know that when I initially tested the instrument, I exceeded that pressure for sure based on the pressure reading of the pump. What happens to the flow cell when it is overpressurized. If I am running the system at 1 mL/min (typical flow rate for previous samples), pressure is already at 6 bar. I thought I can go up to 5 mL/min and be fine, but maybe 5mL/min is just for purging.