GPC - Calibrating a Column (EasiVials)

As stated in previous posts, I am new to GPC. I am trying to perform a calibration using EasiVials and have a set of 12 compounds of different MW at different concentrations (which I assume can be calculated using the stated amount of sample in the vial, the MW and the volume of solvent added). I have 2 questions on that:

I cannot figure out how to create a calibration curve like that shown below (logMW vs Retention time) on chem station.

The only option I can see is Peak Area vs Concentration.

Maybe I am thinking about this wrong, but I would think in order to do a calibration like that I would need the same compound with the same MW (not 12 different ones) at varying concentration as described here (GPCstandardsPrimer_5991-2720EN.pdf (

Also, if I do generate a calibration curve for Peak area vs concentration from my data (I ran duplicate measurements of each red, green, yellow vial), I am not able to use all data sets, I can only generate a calibration curve for one data file including the 4 components in that vial. Can someone point me towards a good description or video that explains this?

Thank you. Bettina

  • Hello Bettina, 

    Glad to hear from you about this. The reason you cannot generate the curve is simply because Chemstation cannot perform GPC analysis. You must have a GPC software designed to calculate molecular weight averages. Depending on your Chemstation you may be able to get an Add on software, otherwise you will need a complete GPC/SEC software. Please call OTS to discuss this further. 


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