hi community we are developing a method on short chain fatty acids in plasma sample, using a DB fatwax UI column on GC-FID. we are using diethyl ether as extraction solvent however our recovery of patient samples are not so good and looking to use Isoproponol (according to some literature) as extraction solvent rather since the diethyl ether (over time seems to convert to acetic acid which in turn interferes with the analysis) my question is would it be safe to use isoproponol as the danger might be that it reacts with the stationary phase? has anyone agree with this or have another suggestion?