Has anyone been having issues with the InfinityLab Poroshell 120 EC-C18 columns (PN 696975-302)?

Has anyone been having issues with the InfinityLab Poroshell 120 EC-C18 columns (PN 696975-302)?  We ordered one, and tried our daily check solution on it but the results did not look like what we normally see.  We also observed that the starting column pressure was lower (165 bar), and went really low (25 bar) as the gradient switched to more organic mobile phase.   We asked for and received a replacement column, and that one worked as expected for about 3 weeks.  Today we saw the same  problem with the daily check.  We checked for leaks, but did not find any.  We have the same column on another instrument that has been functioning normally for months now.  Has anyone else been having issues with these columns?  Any ideas what could be causing this?  Thanks in advance.

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  • Don, 

    The lot we have been using for most of this year is lot B18098.  The lot that did not work as expected was B19135.  Lot B19218 initially worked normally, then the results looked like lot B19135.  I back flushed lot B19218 with 50:50 acetonitrile/water, and the column performed normally (perhaps just had something built up on it).  We have not had any problems with lot B19218 since.  I hope this helps.




  • Hi Mike,


    The factory would like to repeat the experiment with retained production samples for those lots.  They are asking for details about the application.  Since this is a public forum I wouldn't want you to share your application,  phone number or email here.  Could you email lc-column-support@agilent.com with a link to this discussion so they can discuss with you and log it into our support system?





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