ICPOES 5800 wavelength calibration solution orgaic

Hello Agilent Community,

we would like to measure some organic samples (fuels). We neeed the wavelength calibration solution for the organic samples. But on your Internet I did not find for organic. Could you tell me, which one should I order? Thank you very much.

Best regards,


  • Hello Sophia,

    Wavelength calibration for organic samples can be completed using a multi-element standard that has these elements present at a minimum concentration of 5 mg/kg in a suitable solvent (such as Agilent A-solv solvent or kerosene):
    Al, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Mo, Ni, P, Pb, Sn, Ti, Zn.

    The most common way to prepare this is to use one of the Agilent A21 Wear metal standards (with or without K) such as those shown below:
    5190-8603 A21 wear metal standard, Ag, Al, B, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Mo, Na, Ni, P, Pb, Si, Sn, Ti, V, Zn 100 µg/g, in 75 cSt hydrocarbon oil, 100 g
    5190-8604 A21 wear metal standard, Ag, Al, B, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Mo, Na, Ni, P, Pb, Si, Sn, Ti, V, Zn 300 µg/g, 100 g
    5190-8605 A21 wear metal standard, Ag, Al, B, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Mo, Na, Ni, P, Pb, Si, Sn, Ti, V, Zn 500 µg/g, 100 g
    5190-8706 A21 wear metal standard, Ag, Al, B, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Mo, Na, Ni, P, Pb, Si, Sn, Ti, V, Zn 900 µg/g, 100 g

    Hope that helps you,
    Cheers Eric

  • Hi Eric, we have done the wavelength calibration in aqueous solution on our ICP-OES 5800 but will use it for organic analysis now. Do we need to do an organic wavelength calibration before or will the aqueous calibration do?

  • Hi Rick,

    Its a yes and no answer.
    The wavelength calibration does not need to be completed on a daily basis. It is recommended this is performed monthly.
    So if you have recently performed a wavelength calibration, there is no immediate need to repeat this just because you will now perform analysis of organic solvents.
    Remember too that the purpose for the wavelength calibration is to map the location of the actual emission wavelengths across the wavelength range to their corresponding region on the detector. This will not change when you run organic solvents.

    If you were to be running organic solvents regularly from now on, then it would be a good idea to source an organic wavelength calibration standard. This means you do not need to switch between organic and aqueous solutions just to perform this task.

    Hope that helps you,
    Cheers Eric

  • Hi Eric,

    thanks a lot for your reply.

    I saw there are wavelength calibration solution for aqueous samples and the aqueous multi-element standard as well. Does it mean for aqueous samples, I can also use the multi-element standard for the calibration?

    There are 21 elements in the multi-element standard solution, 14 in the wavelength solution? Could you please explain more about it? Thank you very much.

    Best regards


  • Hello Sophia,

    While we do offer a specific wavelength calibration standard, it is also possible to use other multi-element standards to complete the wavelength calibration - but you need to ensure:
    a) That multi-element standard has the key elements present at the right concentration (e.g. make sure K is there at 50ppm)
    b) That the other components in the multi-element standard do not cause any interferences on the emission wavelengths used for the wavelength calibration (you would need to check this from the method editor in the software)

    Using the specific wavelength calibration standard overcomes those potential risks.

    It is also possible to use the wavelength calibration standard (or the concentrate) to prepare the standards you need to calibrate your instrument for daily analysis.

    Hope that helps you,
    Cheers Eric

  • Hey Eric,

    We have a MP-AES we use only for organic are also using that 21 Agilent standard.  When diluted in kero it goes in the machine at about 10 ug/g.  can we use that or would you recommend another concentation?  Machine is fairly new and we haven't actually done the calibration since it was installed. 


  • Hello Michael,

    We would normally prepare the dilution of the 21 element Agilent standard so that the elements were there at a concentration of 5ppm (by weight) or 5ug/g. So the standard you have at 10ug/g is a little higher in concentration than required.

    You could still use it - but it would be better to prepare a lower concentration for the next time you need to do the calibration.

    Hope that helps you,

    Cheers Eric

  • Hi Eric, Sorry for the late reply, the system didn't notify me that you had responded.  Thank  you.   Its good that we we can use the A21+K multi element standard  we already use Agilent Part no 5190-8712 .  Coincidentally the method we have developed has a CCV at 50 ug/g in oil which is diluted 1:10 with solvent, which is rather convenient.  

    We have had some debate about this.  One of the points is that in MP-Expert - Instrument - Calibration - the calibration is looking for Strontium, which isn't present in the A21+K multi element standard, but all the other six lines it is looking for are.     I don't see this a problem because strontium is in the middle of the range at 407.7.  Does this flip the machine out at all?

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