LC column recommendation

Hi,

Find above application guide of SEC/GPC analysis.

Hope, it may help you..

Dear Tee,

"fuel oils" is unfortunately a very generic description of a sample, same for "dimers, trimers, ...". The definition for fuel oil says, that it has a carbon number between 11 and 20, with a possible extension to 26 and is normally a mineral oil.

Some questions need to be answered before there can be any kind of suggestion:

• What kind of fuel oil do you mean? Mineral oil-based, biodiesel-like, etc.?
• As for the oligomers: do you mean acid oligomers and if yes, what are the expected length of the side-chains.
• Are the oligomers linked by in-chain-groups and what are the expected side chain-lengths?
• I assume the sample is only soluble in some organic solvents?
• What would be the estimated max. molecular weight? 
• Does it need to be LC or could it also be GC?

Please give us a little more insight into the analysis and the sample.

Regards,

Norbert

Hi Tee,

I discussed this with a GPC expert and we came out with an article that might help you: 1773 (researchgate.net)

In this article 3 columns with different pore sizes are coupled to get the necessary resolution over a broad MW range.

You can also use a set of PLgel 3 µm MIXED-E columns and here is an example for a polystyrene standard: 5990--7937EN_5989_5672.qxd (agilent.com). Keep in mind that you probably also need two or three columns in series to achieve a good resolution. You can find the part numbers of all these columns in the catalog above.

Regards,

Norbert