I understand for best signal, cell should be almost full as a monolayer in each well. Since cell density is important for the cell mito stress assay, how will too many or too less cells interfere with signal detection?
I understand for best signal, cell should be almost full as a monolayer in each well. Since cell density is important for the cell mito stress assay, how will too many or too less cells interfere with signal detection?
Hello Linh2021,
Thank you for contacting Agilent Cell Analysis Technical Support.
Here is the technical Overview on Optimal Seeding Cell Density for XF assays. Please also visit XF Assay Learning Center for more information.
Please do not hesitate to contact us if you have any questions or concerns. You can reach us by replying to this email or by calling 800-227-9770, option 3, option 8.
Best regards,
Agilent Cell Analysis Technical Support