Cell mitostress assay


I am currently repeating some experiments in a new xFe24 Seahorse analyzer, we used to use a XF24, but unfortunately my cells are not reponding at all to non of the drugs added during the experiment. I am using Agilent kit and also Agilent media, cells are myco free. Do you have any idea of other possible things that I might be missing? Thank you id advance!

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