This Information Applies To: Agilent AriaMx real-time PCR system
Issue
When configuring a plate setup in the AriaMx software, it is crucial to ensure that the selected dyes correspond to the optical modules installed in the instrument.
Background
If there is a mismatch, the following error...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Steps to perform a background calibration on the AriaMx.
Background
A background calibration is required when:
New plasticware is used on the AriaMx
There is a noticeable deviation in the baseline values ( Figure...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx analysis software
Issue
When should the AriaMx diagnostics be run?
Resolution
Agilent Technologies does not provide a specific recommendation regarding when to run instrument diagnostics. However, it is advisable to...
This Information Applies To: AriaMx real-time qPCR system
Issue
The instrument fails to reach the required temperature during a run.
Resolution
Check if the temperature of the environment around the instrument is too low resulting in the instrument failing to heat up as it should. The environment...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx analysis software
Issue
A Copy Number Variation (CNV) experiment is a type of Comparative Quantitation experiment where expected differences are small. Often with fold changes of 0.5 (for one copy loss) to two (for two copy...