This Information Applies To: Agilent OpenLab CDS 2.x
This article outlines the procedure on how to quantify the uncalibrated compounds in OpenLab CDS 2.x when the analytical method requires a fixed response factor.
Uncalibrated compounds can be quantified either with a fixed response factor or using the calibration data of one of the calibrated compounds. Quantitation using a fixed response factor or calibrated compound data is signal-specific.
The unknown compounds must be added as compound in the Processing Method. This procedure requires a built calibration table.
Steps to Follow
The software allows you to quantify an identified compound that is based on a fixed response factor (Manual Factor column). The compound amount is calculated according to the following expression:
Amount = Response * Manual Factor
Manual factor: Fixed response factor
Response: It can be Area, Area%, Height, Height%, Rel. Area, or Rel. Amount
- In the Processing Method, go to Compounds > Calibration (see Figure 1)
- In the Compound Table tab, set the following parameters for every uncalibrated compound:
Mode column: select Manual factor
Manual factor column: Enter the response factor that you want to use (see Figure 1 and Figure 2)
- Click Reprocess All to see the amount of the uncalibrated compound (Figure 2)
Using a manual factor with an ISTD method
If the compound amount is quantified using a fixed response factor and ISTD, the following formula is used:
Relative area = Area/ AreaISDT
Relative height = Height / HeightISTD
The amount is the calculated as follows:
Amount = Relative Area * Manual Factor * AmountISDT
Amount = Relative Height * Manual Factor * AmountISDT
Dependency of manual factor and response factor (RF)
With RF defined as Response per amount (default setting):
RF= 1/ Manual Factor
With RF defined as Amount per response:
RF = Manual Factor
You can also quantify uncalibrated compounds using a calibrated reference compound. See the article Quantitation of Uncalibrated Compounds Using Reference Compounds in OpenLab CDS.
Learn how to effectively operate your Agilent OpenLab CDS Software:
HPLC-OLII-1151e - Agilent OpenLab CDS - Quantification e-learning course available from Agilent Education