This Information Applies To: Agilent OpenLab CDS 2.x
This article outlines the procedure on how to quantify uncalibrated compounds in OpenLab CDS 2.x. using reference compounds in the processing method compound table.
Uncalibrated compounds can be quantified either with a fixed response factor or using the calibration data of one of the calibrated compounds. Quantitation using a fixed response factor or calibrated compound data is signal-specific.
The unknown compounds must be added as compound in the Processing Method. This procedure requires a built calibration table.
Steps to Follow
The calibrated reference compound must be associated to every uncalibrated compound in the processing method:
1. In the Processing Method window, go to Compounds > Calibration (Figure 1)
2. In the Compound Table tab, set the following parameters for every uncalibrated compound
Figure 1: Compound table settings for reference compounds
Calculations are the same as for calibrated compounds. If the reference compound is quantified by an ISTD method, the ISTD is used for the uncalibrated compound in the same way as for the reference compound.
A missing reference peak results in a zero amount of the uncalibrated compound.
Optionally, a correction factor can be entered to multiply the response of the peak before the amount is calculated from the response factor of the reference compound. This factor is entered in the Ref. correction column of the compound table (see Figure 1)
3. Click Reprocess All to see the amount of the uncalibrated compound. (Figure 2)
Figure 2: Indirect quantitation using calibrated compound
You can also quantify uncalibrated compounds using a fixed response factor. See the article Quantitation of Uncalibrated Compounds Using a Fixed Response Factor in OpenLab CDS .
Learn how to effectively operate your Agilent OpenLab CDS Software:
HPLC-OLII-1151e - Agilent OpenLab CDS - Quantification e-learning course available from Agilent Education