Calculating the Peak Resolution for GC Signals with MassHunter Qualitative Analysis

Answer

This Information Applies To: MassHunter qualitative analysis software version B.02.xx and above.

Content

The procedure introduces how to calculate and display the peak resolution for GC signals with MassHunter qualitative analysis software.

Steps to follow

1. Configure the User Interface for GC signals, select GC type in the Configuration > User Interface Configuration > OK.

   
   

2. Load a GC 2D data file. When the warning box appears, select OK, then extract the chromatogram Chromatograms > Extract Chromatograms > Ok.

   
   
   

3. Display the Method Editor by clicking View > Method Editor. In the Method Editor box, click Chromatogram > Integrate (GC) to display the Method Editor box.

   

4. In the Method Editor window Suitability tab, check the Enable system suitability calculations check box, enter the correct Column void time and Column length.

   

   In the same window, select the appropriate Pharmacopeia from the drop-down menu

   

   The Pharmacopeia formula options are:

   Pharmacopeia	Formula
   None	(RT / Sigma) ^2
   USP	16 * (RT / Wb) ^2
   EP / DAB	5.54 * (RT / W50) ^2
   BP	5.545 * (RT/ W50) ^2
   JP	5.555 * (RT / W50) ^2

   Where: RT is the retention time, Sigma is half the peak width at inflection point, Wb is the peak width determined by the tangent method, and W50 is the peak width at 50% of the height of the peak.

5. In the Peak Filters tab, remove all peak filters to properly integrate all the GC peaks.

   

6. On the top of the Method Editor box, click  Integrate Chromatogram. If necessary, adjust the Peak Filters in the Integration settings and re-integrate to integrate only peaks of interest and exclude unwanted peaks and noise.

   

7. Display the Integration Peak List by clicking View menu > Integration Peak List.

   

8. In the Integration Peak List, add the Resolution column: right-click the top bar of the integration peak list and select Add Column > Resolution.

   

9. The Resolution column will be displayed in the Integration Peak List.

   

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