Collection of Automated Electrophoresis Resources

Document created by mnevill Employee on Apr 15, 2020Last modified by shweta.s on Jun 18, 2020
Version 96Show Document
  • View in full screen mode

Here is a "best of" collection of links to manuals and tools for Agilent Automated Electrophoresis products (Bioanalyzer, TapeStation, Fragment Analyzer, ZAG DNA Analyzer, Femto Pulse, Oligo Pro II). For other instruments refer to Collection of Support Documents.

 

Automated Electrophoresis Resources

Agilent Automated Electrophoresis Portfolio

 

 

 

 

2100 Bioanalyzer

 

General

2100 Expert Software User's Guide

2100 Bioanalyzer Maintenance and Troubleshooting Guide

2100 Bioanalyzer Support Tool Box - Download here

              2100 Bioanalyzer Site Preparation Checklist 

              2100 Bioanalyzer Software B.02.10 - Download link

              2100 Bioanalyzer ReadMe file - Software Installation and PC System Requirements

              2100 Bioanalyzer Compliance Solution

              2100 Bioanalyzer Accessories

              2100 Bioanalyzer and TapeStation Systems Consumables

               

Kit Guides

RNA 6000 Nano kit guide

RNA 6000 Pico kit guide

Small RNA kit guide

DNA 1000 kit guide  

DNA 7500 and DNA 12000 kit guide

High Sensitivity DNA kit guide  

Protein 80 kit guide

Protein 230 kit guide

High Sensitivity Protein 250 kit guide

 

Technical and Application Notes 

       DNA

2100 Bioanalyzer DNA Kits Overview

Comparison of Bioanalyzer and UV based Quantitation of QPCR Standards

2100 Bioanalyzer for Basmati Rice Authenticity Testing

Sample QC using Bioanalyzer and TapeStation for NGS Solutions

FFPE sample QC using Bioanalyzer 

NGS library prep QC using 4150 TapeStation and 2100 Bioanalyzer

Comparison of DNA Assays Using the 4200 TapeStation System and 2100 Bioanalyzer System

RNA

              2100 Bioanalyzer RNA Kits Overview

              Small RNA Analysis

              RNA Integrity Number (RIN)

              Plant RNA RIN

              Insect RNA RIN

              Quantitation Comparison of total RNA using Bioanalyzer, RiboGreen and UV

              Quality Control of Guide RNA for CRISPR

              DV200 Determination Tool and DV200 Technical Overview

Protein

2100 Bioanalyzer Protein Kits Overview

Protein Assays Portfolio with Data

Analysis of Glycosylated Proteins on the Bioanalyzer 

 

Maintenance and Troubleshooting

      2100 Bioanalyzer Maintenance and Troubleshooting Guide -  RNA, DNA, Protein, Instrument Communication Related Issues

      Running Hardware Diagnostics

      2100 Bioanalyzer Support Tool Box - Download here

      2100 Bioanalyzer : How To Video - Priming Station Maintenance

      2100 Bioanalyzer : How to Video - Prepare a Chip 

      2100 Bioanalyzer : How To Video - Exchanging Cartridges

      2100 Bioanalyzer: How To Video - Electrode Cartridge Maintenance

 

 

4200 TapeStation

 

General

4200 TapeStation User Manual

4200 TapeStation Site-Preparation Checklist

4200 TapeStation Compliance Solutions

4200 Software 3.2 - Download here

4200 Software ReadMe file - Installation and PC Requirements

LIMS Integration of the 4200 TapeStation System

4200 TapeStation Consumables and Reagents

4200 TapeStation Accessories

 

Kit Guides

DNA

D1000 ScreenTape assay guide

HSD1000 ScreenTape assay guide

D5000 ScreenTape assay guide

HSD5000 ScreenTape assay guide

Genomic DNA ScreenTape assay guide

Cell-free DNA ScreenTape assay guide

RNA

RNA ScreenTape assay guide 

HSRNA ScreenTape assay guide

 

Technical and Application Notes

DNA

DNA Integrity Number (DIN)

Genomic DNA ScreenTape Data Sheet

D5000 ScreenTape and High Sensitivity D5000 ScreenTape Assays Data Sheet

Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays on 4200 TapeStation System

Agilent D1000 ScreenTape and High Sensitivity D1000 ScreenTape Data Sheet

Performance of the D1000 and High Sensitivity D1000 ScreenTape Assays on 4200 TapeStation System

Sample QC during Agilent SureSelectQXT WGS library preparation

Sample QC using Bioanalyzer and TapeStation for NGS Solutions.

QC of Magnis SureSelect Workflow using Agilent Automated Electrophoresis Platforms

Cell-free DNA Data Sheet

Performance Characteristics of Cell-free DNA ScreenTape Assay 

Sample QC with Cell-free DNA ScreenTape Assay  

Comparison of DNA Assays Using the 4200 TapeStation System and 2100 Bioanalyzer System

RNA

RIN verses RINe

Performance of the RNA ScreenTape Assays on the 4200 TapeStation System

DV200 Evaluation with RNA ScreenTape Assays

      

Maintenance and Troubleshooting

      4200 TapeStation Troubleshooting (starting page 153)

      4200 TapeStation Troubleshooting Software (starting page 155)

      4200 TapeStation Troubleshooting DNA Applications (starting page 162)

      4200 TapeStation Troubleshooting RNA Applications (starting page 183)

      4200 TapeStation Troubleshooting Instrument Communication (starting page 196)

      Running Hardware Diagnostics and Collecting Troubleshooting Information (starting page 205)

      4200 TapeStation Good Measurement Practices (starting page 81)   

      Annual Preventive Maintenance (PM) Details (starting page 130) (PM to be executed by Agilent certified engineer)

      Exchange the Piercing Needle (starting 131) (done during annual PM and can be done by users, if needed) 

      Cleaning the Instrument (starting page 136) 

      Engage the Transportation Lock (starting page 139)

      Assembling Nest covers steps (starting page 145and also listed in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC)

      Check correct Insertion of lower nest cover (page 152and animation in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC

 

4150 TapeStation

 

General

4150 TapeStation User Manual

4150 TapeStation Site-Preparation Checklist

              4150 Compliance Solutions

4150 Software 3.2 - Download here

4150 Software ReadMe file - Installation and PC Requirements

LIMS Integration of the 4150 TapeStation System

4150 TapeStation Consumables and Reagents

 

Kit Guides

       DNA

D1000 ScreenTape assay guide

HSD1000 ScreenTape assay guide

D5000 ScreenTape assay guide

HSD5000 ScreenTape assay guide

Genomic DNA ScreenTape assay guide

Cell-free DNA ScreenTape assay guide

       RNA

       RNA ScreenTape assay guide 

HSRNA ScreenTape assay guide

 

Technical and Application Notes

DNA

DNA Integrity Number (DIN)

Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays on 4150 TapeStation System

Performance of the D1000 and High Sensitivity D1000 ScreenTape Assays on 4150 TapeStation System

Performance of the Genomic DNA ScreenTape Assay on 4150 TapeStation System

QC of Magnis Sureselect Workflow using Agilent Automated Electrophoresis Platforms

Sample QC using Bioanalyzer and TapeStation for NGS Solutions.

NGS library QC using 4150 TapeStation and 2100 Bioanalyzer at different steps

Cell-free DNA Data Sheet

Performance Characteristics of Cell-free DNA ScreenTape Assay 

Sample QC with Cell-free DNA ScreenTape Assay  

RNA

RIN verses RINe

Performance of the RNA and High Sensitivity RNA ScreenTape Assays on 4150 TapeStation System

DV200 Evaluation with RNA ScreenTape Assays

 

Maintenance and Troubleshooting

        4150 TapeStation Troubleshooting Software (starting page 169)

 4150 TapeStation Troubleshooting DNA Applications (starting page 180)

 4150 TapeStation Troubleshooting RNA Applications (starting page 202)

 4150 TapeStation Troubleshooting Instrument Communication (starting page 215)

 Running Hardware Diagnostics on 4150 TapeStation (starting page 115)

 Collecting 4150 TapeStation Troubleshooting Information (starting page 224)

 4150 TapeStation Good Measurement Practices (starting page 81)

 4150 TapeStation Maintenance Details (starting page 125)

 Exchange the Piercing Needle (starting page 129) (done during annual PM and can be done by users, if needed)

 Exchange Electrode Cartridge (starting page 137) (done during annual PM and can be done by users, if doing self PM)

 Cleaning the Instrument (starting page 148)

 Engage the Transportation Lock (starting page 149)

        Assembling and Inserting Nest Covers steps (starting page 158and also listed in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC

        Check Correct Insertion of Lower Nest Cover (page 165and animation in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC) 

 

2200 TapeStation

 

General

2200 TapeStation User Manual

2200 TapeStation Software A.02.02 SR1 - Download here

2200 Software ReadMe file - Installation and PC Requirements

2200 TapeStation Accessories

 

Kit Guides

      DNA 

       D1000 ScreenTape assay guide

HSD1000 ScreenTape assay guide

D5000 ScreenTape assay guide

HSD5000 ScreenTape assay guide

Genomic DNA ScreenTape assay guide

Cell-free DNA ScreenTape assay is NOT available for 2200 Tapestation

       RNA

       RNA ScreenTape assay guide

       HSRNA ScreenTape assay guide

 

 Technical and Application Notes

DNA

DNA Integrity Number (DIN)

Performance of the D1000 and High Sensitivity D1000 ScreenTape Assays on 2200 TapeStation System

Analysis of genomic DNA on 2200 Tapestation

RNA

RIN verses RINe

Performance of the RNA ScreenTape Assays on the 2200 TapeStation System

DV200 Evaluation with RNA ScreenTape Assays

 

Maintenance and Troubleshooting

      2200 TapeStation Troubleshooting Manual

      2200 TapeStation Troubleshooting Instrument Communication (starting page 23)

      2200 TapeStation Troubleshooting DNA Applications (starting page 39)

      2200 TapeStation Troubleshooting RNA Applications (starting page 67)

      2200 TapeStation Troubleshooting Error Messages (starting page 93)

      Collecting 2200 TapeStation Troubleshooting Information (starting page 34)

      2200 TapeStation Good Measurement Practices

      2200 TapeStation Essential Measurement Practices (starting page 5)

      2200 TapeStation Maintenance Details (starting page 85)

      Exchange the Piercing Needle (starting page 87; done during annual PM and can be done by users, if needed)

 

 

Fragment Analyzer

 

General

Fragment Analyzer User Manual

 Fragment Analyzer Controller Updater Software 1.2.0.11 

Fragment Analyzer Controller Software 1.2.0.12 and Fragment Analyzer Drivers

ProSize (3.0.1.6) Data Analysis Software

FFPE DV200 Calculation Method/Configuration File 

CRISPR Plug-in Configuration File

Fragment Analyzer Consumables, Array Options

Capillary Array Uninstalling, Unpacking and Installing

 Nucleic Acid analysis using 5200, 5300, and 5400 Fragment Analyzer Systems

 Array Formats for Parallel CE Instruments

 

Kit Guides

       DNA Quantitative

HS Large Fragment 50 kb Kit- DNF-464-0500

Large Fragment Kit - DNF-492-0500

Large Fragment Kit - DNF-492-1000

HS Large Fragment Kit - DNF-493-0500

HS Large Fragment Kit - DNF-493-1000

Genomic DNA 50 kb Kit - DNF-467-0500

HS Genomic DNA 50 kb Kit - DNF-468-0500

HS Genomic DNA Kit - DNF-488-0500

HS Genomic DNA Kit - DNF-488-1000

NGS Fragment Kit - DNF-473-0500

NGS Fragment Kit - DNF-473-1000

HS NGS Fragment Kit - DNF-474-0500

HS NGS Fragment Kit - DNF-474-1000

Small Fragment Kit - DNF-476-0500

HS Small Fragment Kit  - DNF-477-0500

HS Small Fragment Kit  - DNF-477-1000

       DNA Qualitative

dsDNA 905 Reagent Kit - DNF-905-K0500

dsDNA 905 Reagent Kit - DNF-905-K1000

dsDNA 935 Reagent Kit - DNF-935-K0500

dsDNA 935 Reagent Kit - DNF-935-K1000

dsDNA 910 Reagent Kit- DNF-910-K0500

dsDNA 910 Reagent Kit - DNF-910-K1000

dsDNA 915 Reagent Kit - DNF-915-K0500

dsDNA 915 Reagent Kit - DNF-915-K1000

dsDNA 920 Reagent Kit  - DNF-920-K0500

dsDNA 930 Reagent Kit - DNF-930-K0500

dsDNA 930 Reagent Kit - DNF-930-K1000

CRISPR Discovery Gel Kit - DNF-910-K1000CP

Plasmid DNA Kit - DNF-940-K0500

RNA quantitative

Small RNA Kit - DNF-470-0275

RNA Kit - DNF-471-0500

RNA Kit - DNF-471-1000

HS RNA Kit - DNF-472-0500

HS RNA Kit - DNF-472-1000

 

 Technical and Application Notes

Quantification using 5200 Fragment Analyzer System

Sizing using 5200 Fragment Analyzer System

DNA

Genomic DNA Quality assessment using 5200 Fragment Analyzer 

QC of cell free DNA using 5200 Fragment Analyzer

Resolution (up to 3 bp) using dsDNA 905 kit (DNF-905) and HS small Fragment Kit (DNF-477) on 5200/5300 Fragment Analyzer

RNA 

Eukaryotic Total RNA QC on 5200 Fragment Analyzer using HS RNA Kit (DNF‑472) and RNA Kit (DNF-471)

Single-Guide RNA (CRISPR) QC on 5200/5300/5400 Fragment Analyzer using HS RNA Kit (DNF- 472) and Small RNA Kit (DNF-470)

Comparison of HS Small Fragment Kit (DNF‑477) and HS NGS Fragment Kit (DNF‑474) on the Agilent 5200 Fragment Analyzer

QC of IVT RNA on 5200 Fragment Analyzer using HS RNA Kit (DNF-472) and RNA Kit (DNF‑471) 

Plant RNA Degradation Detection on 5200 Fragment Analyzer using HS RNA Kit (DNF-472) and RNA Kit (DNF-471) 

 

Maintenance and Troubleshooting

Preventative Maintenance Schedule

Capillary Array Alignment

Capillary Array Cleaning

Syringe Replacement

Collecting Troubleshooting Information - Error Reports and Zipped Data Files

 

ZAG DNA Analyzer

 

General

Agilent ZAG Analyzer Manual

ZAG DNA Analyzer Brochure

ZAG Controller Updater Software 1.2.0.11 

ZAG Controller Software 1.2.0.11 and Drivers

ZAG Analyzer Prosize Analysis Software 3.0.1.6

ZAG Capillary Array Uninstall, Unpack and Install

ZAG Consumables, Analysis Kits and Array options

Array Formats for Parallel CE Instruments

 

Kit Guides 

ZAG 105 dsDNA Kit (1-500 bp) - ZAG-105-5000

ZAG 110 dsDNA Kit (35–5000 bp) - ZAG-110-5000

ZAG 130 dsDNA Kit (75 - 20000 bp) - ZAG-130-5000

ZAG 135 dsDNA Kit (1–1500 bp) - ZAG-135-5000

 

Technical and Application Notes

      DNA

             DNA Fragment Sizing with the ZAG

             Genotyping using ZAG

             Sizing Resolution on ZAG using 105 dsDNA Kit

 

Maintenance and Troubleshooting

ZAG Preventative Maintenance Schedule

ZAG Capillary Array Uninstall, Unpack and Install

ZAG Capillary Array Alignment

ZAG Capillary Array Cleaning

Syringe Replacement

Collecting Troubleshooting Information - Error Reports and Zipped Data Files

                

Femto Pulse

 

General

Femto Pulse User Manual

 Femto Pulse System Brochure

Femto Pulse Installer with Drivers 1.0.0.32

 Femto Pulse ProSize Data Analysis 3.0.1.6

Femto Pulse Kits

Array Formats for Parallel CE Instruments

 

Kit Guides 

DNA

Genomic DNA 165 kb Kit - FP-1002-0275

Ultra Sensitivity NGS Kit - FP-1101-0275

55 kb BAC Kit - FP-1003-0275

RNA

Ultra Sensitivity RNA Kit - FP-1201-0275

 

Technical and Application Notes

       DNA

Quantify and Qualify DNA Fragments using FP-1002 gDNA 165 Kb Analysis Kit on the Femto Pulse system

Genomic DNA QC using Genomic DNA 165 kb Kit (FP-1002-0275) on the Femto Pulse System

GQN Assessment of Genomic DNA using Genomic DNA 165 kb Kit (FP-1002-0275) on the Femto Pulse System

Ultra-Sensitivity NGS Analysis Kit (FP-1101) on the Femto Pulse System

Cell free DNA QC using Ultra Sensitivity NGS Kit (FP-1101) on the Femto Pulse System

RNA

Ultra-Sensitivity RNA Analysis Kit (FP-1201) and the Femto Pulse System

 

Maintenance and Troubleshooting

      Femto Pulse User Manual - Capillary Array Uninstall, Install (Chapter 9)

      Syringe Replacement

      Collecting Troubleshooting Information - Error Reports and Zipped Data Files

 

Oligo Pro II

 

      General

      Oligo Pro II Controller Software and Oligo Pro II Controller Drivers

Oligo Pro II Data Analysis Software Version 1.0.0.0

      Oligo Pro II Instrument User Manual

             Oligo Pro II Data Analysis Software User Manual

      Oligo Pro II Reagent Structure

      Array Formats for Parallel CE Instruments

Oligonucleotide Purity Assessment on Oligo Pro II System

 

 Maintenance and Troubleshooting

Oligo Pro II Capillary Array Uninstall and Install

Collecting Troubleshooting Information - Error Reports and Zipped Data Files

 

Keywords: troubleshootingmanualarrayuser manual4200210041502200520053005400oligo pro ii2200 tapestation5200 fragment analyzer5300 fragment analyzer5400 fragment analyzer

zag dna analyzerarray installarray uninstall4150 tapestation4200 tapestation2100 bioanalyzererror reportevent reportzipped data filezippedlog filessupport package

Attachments

    Outcomes