Currently, we find a method using HPLC from Waters so that we plan to buy a column from Waters. I don't know if this column can fit into the Agilent machine
Currently, we find a method using HPLC from Waters so that we plan to buy a column from Waters. I don't know if this column can fit into the Agilent machine
Hi Yangbei,
yes, those columns would fit into an Agilent LC instrument. You need to check the fitting of the column against the ones of your connection tubing on the Agilent machine, sometimes another ferrule/fitting needs to be used. There is a webinar about this here.
But have you had a look at our two application notes about multi-vitamin samples?
Both done on Poroshell columns and very fast, Vitamin B5 eluting between 3.5 and 3.7 minutes.
Regards,
Norbert
Hi Dr. Norbet
Thanks for your reply. But I do have a question about the second paper you refer to me (Analysis of water-soluble vitamins from multivitamin tablets for nutrition labeling). I can't find the sample preparation method. In that paper, it uses the multivitamin tablet so that the vitamin content can fully dissolve into the water. What about the sample with hard texture? Do I need to use acid to break the protein and then fully extract the vitamin B5 in the sample?
Hi Dr. Norbet
Thanks for your reply. But I do have a question about the second paper you refer to me (Analysis of water-soluble vitamins from multivitamin tablets for nutrition labeling). I can't find the sample preparation method. In that paper, it uses the multivitamin tablet so that the vitamin content can fully dissolve into the water. What about the sample with hard texture? Do I need to use acid to break the protein and then fully extract the vitamin B5 in the sample?
Hi yangbei,
the sample preparation is described on page 3 under the headline "Sample Preparation", the tablets are dissolved in 200 mL water under sonication with an added amount of 0.25M-NaOH and the solution is filtered through a syringe filter with 0.25 µm pore size.
If your sample is harder you have to finely grind it first, put it in the water and sonicate longer.
Regards,
Norbert
Hi Dr. Norbet
Thank you very much