I would like to measure the Vitamin B5 content in the raw material. Can anyone tell me how to measure vitamin B5 by using the HPLC-UV and which column should we choose?

Currently, we find a method using HPLC from Waters so that we plan to buy a column from Waters. I don't know if this column can fit into the Agilent machine 

Parents Reply
  • Hi yangbei,

    the sample preparation is described on page 3 under the headline "Sample Preparation", the tablets are dissolved in 200 mL water under sonication with an added amount of 0.25M-NaOH and the solution is filtered through a syringe filter with 0.25 µm pore size.

    If your sample is harder you have to finely grind it first, put it in the water and sonicate longer.



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