# Automatic Signal/Noise calculation

Hello,

I'm using Open Lab 2 to calculate the signal-to-noise ratio.
When I choose the option "automatic (On blank recommended for EP", on which blank of the sequence is the signal to noise calculated? Over what time range is the calculation made? Does it take into account the injection peak?

Parents
• Hello,

If you search the help, you can find more details on the SN calculations. For automatic calculations the last blank in the results set prior to the injection is used. So, if you run blanks throughout the sequence a blank is used for the injections after it until another blank is run. The auto time range calculation is shown below, it uses the RT of the peak in the calculation.

Marty

An automatically determined time region is calculated according to one of the following algorithms:

• If the reference signal is not long enough

(EndTime - StartTime < 20*W50)

• StartTime = starttime (of reference signal), and

• EndTime = endtime (of the reference signal)

• If the reference signal is long enough, but the peak is situated too close to the starttime

(tR-10*W50 < starttime of the reference signal)

• StartTime = starttime (of reference signal), and

• EndTime = StartTime + 20*W50

• If the reference signal is long enough, but the peak is situated too close to the endtime

(tR+10*W50 > endtime of the reference signal)

• EndTime = endtime (of the reference signal), and

• StartTime = EndTime - 20*W50

• If the reference signal is long enough, and the peak is situated far enough away from starttime and endtime of the reference signal

(tR-10*W50 > starttime, tR+10*W50 < endtime)

• StartTime = tR - 10*W50, and

• EndTime = tR + 10*W50

where

tR is the retention time, and

W50 is the peak width at half height.

• Hi Martin, I am using the setting as below (All peaks, ASTM, Automatic). Unfortunately, the S/N for my peak (Toluene) is not calculated and the warning of " At least one peak where noise ASTM on blank with automatic range could not be computed"  is appeared. My blank (Methanol) has not peaks other than the solvent peak. The blank injection is just before the suitability injection.

What could be the reason and how to solve this?

Thank you

Kris