Curve coefficient didn't correspond to the average.


First, I want to show how I perfomed my 1 level calibration curve. 

We saw that I asked for the average when several calibration standards are used and contained the same target compound. 

We do have the TFT in each of my 4 calibration standards. 

We can see it at my section filtered for Cal std only. 

What's weird here it's the first RF calculated is good (1083376/9.8 = 110549) but all the other ones are wrong. 

If we take the 2nd one and did the same calculation (1110109/9.8= 113276) and the software gave 111912. 

I don't know where this 111912 result is coming from. 

Here my injection list that shows how I performed my calibration.

Is it because my Cal.Std are not injected one immediately after another? 

I can see on my calibration curve that I has four point but the coefficient a given in the table is coming from the first injection only and not the average like I choose on the method process. 

We can't see that the a coeffiecient on the curbe at 110549 is coming from the first injection. 

So it seems that the Curve calculation is not with the average of the four peaks.

Why ?

thank you

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