I am working with a method that uses narrow standard, polynomial curve (3rd order) as calibration.
Calibration curve is being obtained with 0.9998 of coefficient of determination and stdev of 0.027.
OpenLab calculates Mp for my sample as 37759. When I use the obtained calibration curve and the RT to calculate manually, Mp = 37126.
Can someone please inform me why I don't obtain the same result while using the software to calculate result for sample compared to manual calculation?
Does openlab applies any correction based on Residuals to calculate sample results?
Thank you for your help.
All the best,