# Statistics uniformity of content

Hi,

My company uses the following sequence set-up for uniformity of content:

- Bracket1

- sample 1

- sample 2

- sample 3

- sample 4

- Bracket 2

- sample 5

- sample 6

- sample 7

- sample 8

- bracket 3

- sample 9

- sample 10

- Bracket 4

The assay of sample 1 - 4 will be calculated using average bracket 1 + 2. Sample 5 - 8 will be calculated using average bracket 2 + 3.

The calculations are fine for assay calculation, only struggle is the average %, stdev an %RSD over n=10 sample assay results.

What is the best practice to use the aggregates?