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All things being equal with respect to hardware, column, sample etc., would GC data collected via Open Lab, Chemstation and Mass Hunter all yield the same peak areas?

squawkbert

We are actually running a new 7890B FID on Empower 3 and are getting really low area counts as compared to the I/OQ areas collected by an Agilent field service rep. using a laptop with Mass Hunter.

 

Waters says that despite their having had a problem with a previous version of the ICF in play here, the problem did not extend to Mass Hunter. Only Chemstation and Open Lab had the issue. I am inclined to call "BS" on this as we are still seeing very low area counts for what should be the same injections made using the same everything (except for data system). Our I/OQ docs show nearly 3 million area counts for the C15 and C16 peaks in the FID MDL test mix via Mass Hunter and we're getting just over 300 area counts in Empower 3. I know that this is not inherently a bad thing as Empower also allows for 5 decimal places but we're only getting about half the S/N in Empower as was reported in the I/OQ doc.

 

Any suggestions appreciated...

Thanks in advance,

DR

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    Hello,

     

    To cut to the chase: No, peak areas calculated in MassHunter will not be equal to the peak areas calculated in GC/OpenLAB ChemStation software.

     

    GC ChemStation and OpenLAB ChemStation use response units of pA for FID data whereas the response axis for GC-FID data collected in MassHunter or MSD ChemStation is unit-less. Since the software products are not using the same response units then the peak area calculated by these products will be significantly different, even if they are integrating the same chromatographic data.

  • 0 in reply to ryoboyle

    Thanks for the information. So its it fair to say that the pA units displayed on the y-axis of the I/OQ report plots are “derived”?

     

    Regards,

    Dan

  • 0 in reply to squawkbert

    I cannot say for certain about the pA units on the I/OQ report. Agilent OQ reports are generated in the Agilent Compliance Engine and I am not familiar with that software.

     

    Generally speaking, when comparing data acquired/analyzed in multiple different software products you want to compare the calculated amount/concentration results as opposed to the peak integration results. As long as the quantification method is properly calibrated and the peak integration is reproducible then similar results should be calculated by the different software products.

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  • 0 in reply to squawkbert

    I cannot say for certain about the pA units on the I/OQ report. Agilent OQ reports are generated in the Agilent Compliance Engine and I am not familiar with that software.

     

    Generally speaking, when comparing data acquired/analyzed in multiple different software products you want to compare the calculated amount/concentration results as opposed to the peak integration results. As long as the quantification method is properly calibrated and the peak integration is reproducible then similar results should be calculated by the different software products.

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